Abstract Aspergillus ostianus Wehmer was a new record to the Mycological Laboratories in Egypt, isolated form cabbage seeds and tested for aflatoxins and ochratoxin A production. Aflatoxins B1 and G1 and ochratoxin A were demonstrated in the crude extracts of Aspergillus ostianus cultivated on different media for mycotoxins production. Both of essential oil and ethanol extract of clove were tested in order to control Aspergillus ostianus. The minimum inhibitory concentration (MIC) was 46.77 and 500 mg/ml for essential oil and ethanol extract, respectively. Also, qualitative phytochemical screening of the extracts and their chemical components using GC/MS analysis were determined.